RNA

Part:BBa_K3001009:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lba crRNA targeting GFP with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A T7 promoter was added to allow in vitro transcription using T7 polymerase and allow expression in E. coli BL21(DE3) or Rosetta (DE3) cells. A double terminator was also added to signal transcription termination.

Source

Designed by Laura Keffer-Wilkes based off of designs by Munich 2017. Synthesized by IDT.

References